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KMID : 1140120080130040276
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Cancer Prevention Research
2008 Volume.13 No. 4 p.276 ~ p.283
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Curcumin Specifically Inhibits DPPH Radical and Matrix Metalloproteinase-9 in Human Fibrosarcoma Cells
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Lee Soo-Jin
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Abstract
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The present study is to investigate the inhibitory effect of curcumin derived from rhizome of Curcuma longa on matrix metalloproteinases (MMPs) and oxidative stress in human fibrosarcoma cells (HT1080). It has been paid an attention to a variety of physiological activities such as prevention of cancer, anti-inflammatory activity and antioxidant effect. However, the mechanism of its action remains unclear in human. Thus, for the first time the direct scavenging effects of curcumin on DPPH radical, superoxide and hydroxyl radical related to oxidative strsess were evaluated using electron spin resonance spectroscopy. Furthermore, its antioxidant effect was monitored in human fibrosarcoma cells exposed to hydrogen peroxide. In addition, its inhibitory effects on the activity and expression of MMP-2 and -9 related to metastasis were determined using gelatin zymography and western blot. In this study, it was observed that curcumin exhibited the highest inhibitory effect on DPPH radical in reactive oxygen species, and blocked DNA oxidation induced by hydroxyl radical. The oxidative stress in HT1080 cells exposed to hydrogen peroxide was also repressed in the presence of curcumin at 1μM. Moreover, it specifically inhibited both activity and expression of MMP-9. These findings confirm that curcumin could be developed as an excellent agent for cancer prevention.
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KEYWORD
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Curcumin, Electron spin resonance spectroscopy, Reactive oxygen species, Matrix metalloproteinase, HT1080
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